Fig. 6. ZNF281 promotes CRC cell growth and migration through the WNT/β-catenin pathway. (A) The ZNF281, β-catenin and c-myc mRNA expression levels in CRC cells transfected with vector or sh-ZNF281 were analyzed using reverse transcription-quantitative polymerase chain reaction analysis; GAPDH served as an internal control. (B) Western blot was performed to detect β-catenin and c-myc expression after ZNF281 knockdown, and GAPDH was used as an internal control. (C) Western blotting analysis in SW480 and HCT116 cells in which ZNF281 was knocked down after treatment with 10 μΜ XAV939. Mean ± SEM, t-test, *P<0.05, **P<0.01, ***P<0.001.